• 经测试应用WB, ICC/IF, IHC-Pmore details
• 种属反应性
  与反应: Mouse, Rat, Human
• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SNRPD2 aa 50 to the C-terminus. The exact sequence is proprietary.
  Database link: P62316

• 阳性对照
  • WB: HepG2, MCF7, A549, HeLa, Mouse brain, Mouse spleen, Rat brain, Mouse spleen, C6, RAW 264.7 and PC12 lysates. IHC: Human and Rat kidney tissues. ICC/IF: HeLa and MCF7 cells.
• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
• 浓度100 µl 浓度为 1.626 mg/ml 
  
• 纯度Protein A purified
• 克隆单克隆
• 克隆编号EPR16762
• 同种型IgG
• 研究领域
  • Epigenetics and Nuclear Signaling
   
  • DNA / RNA
   
  • RNA Processing
   
  • Splicing

应用

靶标

• 功能Required for pre-mRNA splicing. Required for snRNP biogenesis.
• 序列相似性Belongs to the snRNP core protein family.
• 细胞定位Nucleus.
• Information by UniProt
• 数据库链接
  • Entrez Gene: 6633 Human
  • Entrez Gene: 107686 Mouse
  • Entrez Gene: 680309 Rat
  • Omim: 601061 Human
  • SwissProt: P62316 Human
  • SwissProt: P62317 Mouse
  • Unigene: 515472 Human
  • Unigene: 29135 Mouse

  see all
• 别名
  • Sm-D2 antibody
  • small nuclear ribonucleoprotein D2 antibody
  • Small nuclear ribonucleoprotein D2 (RCG54604) antibody

  • small nuclear ribonucleoprotein D2 polypeptide (16.5kD) antibody
  • small nuclear ribonucleoprotein D2 polypeptide 16.5kDa antibody
  • SMALL NUCLEAR RIBONUCLEOPROTEIN POLYPEPTIDE D2; SNRPD2 antibody
  • Small nuclear ribonucleoprotein Sm D2 antibody
  • SMD2 antibody
  • SMD2_HUMAN antibody
  • snRNP core protein D2 antibody
  • SNRPD1 antibody
  • Snrpd2 antibody
  • SNRPD2 small nuclear ribonucleoprotein D2 polypeptide 16.5kDa antibody

  hide

Anti-SNRPD2 antibody [EPR16762] 图像

• 

  Western blot - Anti-SNRPD2 antibody [EPR16762] (ab198296)
  All lanes : Anti-SNRPD2 antibody [EPR16762] (ab198296) at 1/20000 dilution
  
  Lane 1 : HepG2 cell lysate
  Lane 2 : MCF7 cell lysate
  Lane 3 : A549 cell lysate
  Lane 4 : HeLa cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
  developed using the ECL technique
  
  Predicted band size : 14 kDa
  Observed band size : 14 kDa
  
  
  Exposure time : 30 seconds

  Blocking/Dilution buffer: 5% NFDM/TBST.

• 

  Western blot - Anti-SNRPD2 antibody [EPR16762] (ab198296)
  Lanes 1 - 6 : Anti-SNRPD2 antibody [EPR16762] (ab198296) at 1/2000 dilution
  Lane 7 : Anti-SNRPD2 antibody [EPR16762] (ab198296) at 1/20000 dilution
  
  Lane 1 : Mouse brain lysate
  Lane 2 : Mouse spleen lysate
  Lane 3 : Rat brain lysate
  Lane 4 : Rat spleen lysate
  Lane 5 : C6 cell lysate
  Lane 6 : RAW 264.7 cell lysate
  Lane 7 : PC12 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
  developed using the ECL technique
  
  Predicted band size : 14 kDa
  Observed band size : 14 kDa
  
  
  Exposure time : 30 seconds

  Blocking/Dilution buffer: 5% NFDM/TBST.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPD2 antibody [EPR16762] (ab198296)

  Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SNRPD2 with ab198296 at 1/600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm and nucleus staining on Human kidney tissue is observed. Counter stained with Hematoxylin.

  Negative control Using PBS instead of primary antbody, secondary ab is Goat Anti-Rabbit IgG H&L (HRP)

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPD2 antibody [EPR16762] (ab198296)

  Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling SNRPD2 with ab198296 at 1/600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm and nucleus staining on Rat kidney tissue is observed. Counter stained with Hematoxylin.

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