Bme18I内切酶-RNAi技术-试剂-生物在线
Bme18I内切酶

Bme18I内切酶

商家询价

产品名称: Bme18I内切酶

英文名称: Bme18I

产品编号: HZ2076

产品价格: 0

产品产地: 中国/上海

品牌商标: 沪震生物

更新时间: 2023-08-17T10:24:20

使用范围: null

上海沪震实业有限公司
  • 联系人 : 鲍丽雯
  • 地址 : 上海市闵行区闵北路88弄1-30号第22幢AQ136室
  • 邮编 : 200612
  • 所在区域 : 上海
  • 电话 : 139****0749
  • 传真 : 021-60345367
  • 邮箱 : www.shzbio.net

 Bme18I内切酶

中文名称:Bme18I内切酶
英文名称:Bme18I
产品规格:500U
储存条件:冷冻(-20℃)

技术规格
 

Unit definition One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sites G↑GWCC
来源 Bacillus megaterium 18
Assayed on Lambda DNA
Optimal buffer O(50mMTris-HCl(pH 7.6 at 25℃);10 mM MgCl2;100 mM NaCl;1 mM DTT.)
Optimal temperature 37℃
Storage conditions 10 mM Tris-HCl(pH 7.5);100 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;100 μg/ml BSA;50%glycerol.Store at -20℃.
Ligations After 10-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut.
Non-specific hydrolisis No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 20 u.a.of enzyme for 16hoursat 37℃.
Methlation effect Cleaved of DNA is impaired by overlapping dcm-methylation(CmCWGG):GGWCCWGG.
Thermal inactivation enzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible Ends AflI,AvaII,Eco47I,PspO3I^,SinI,Vpak11AI^

 

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